Inter-individual variability values were normalized such that variability values for nlp-29 in wild type = 1 (bcSi9 or zcIs13). To estimate inter-individual variability in gene expression, the coefficient of variation was calculated from individual mRNA levels obtained from normalized Ct values using the delta Ct method. (F) Inter-individual variability in gene expression of target genes in bcSi9 and zcIs13 in both wild type (+/+) and fzo-1(tm1133). Values indicate means of relative HSP-6 expression (HSP-6/TUB) ± SD, n = 2. For each genotype, 100 L4 larvae were harvested per experiment for western blot analysis. (E) Quantifications of western blot analysis of endogenous HSP-6 levels in wild-type (+/+), spg-7(ad2249), fzo-1(tm1133) or drp-1(tm1108) using anti-HSP-6 antibodies. Values indicate means ± SD of ≥4 independent measurements. Each dot represents quantification of 15–20 L4 larvae.
(D) Quantifications of fluorescence images of panel B (P hsp-6 gfp (bcSi9)) are shown. Values indicate means ± SD of ≥5 independent measurements. (C) Quantifications of fluorescence images of panel A (P hsp-6 gfp (zcIs13)) are shown.
(B) Brightfield (upper panel) and fluorescence images (lower panel) of L4 larvae expressing P hsp-6 gfp (bcSi9) in wild type (+/+), spg-7(ad2249), fzo-1(tm1133) or drp-1(tm1108). (A) Brightfield (upper panel) and fluorescence images (lower panel) of L4 larvae expressing P hsp-6 gfp (zcIs13) in wild type (+/+), spg-7(ad2249), fzo-1(tm1133) or drp-1(tm1108). S1 Fig: Comparison of expression levels and inter-individual variability of multi-copy P hsp-6 mtHSP70 gfp (zcIs13) and single-copy integrated P hsp-6 mtHSP70 gfp (bcSi9) transgenes.
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